Methods for producing proteins by using cell-free protein...

Details Methods for producing proteins by using cell-free protein... Methods for producing proteins by using cell-free protein...

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 1/00 (2006.01) C07K 2/00 (2006.01) C07K 14/82 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2363461

A method for producing a protein suitable for X-ray crystallographic analysis, in a cell-free protein synthesis system comprising a cell-free extract, a nucleic acid coding for said protein, and amino acids for the substrate of said protein, wherein said amino acids comprises at least one amino acid comprising a heavy atom, and wherein the introduced rate of said amino acid comprising the heavy atom into the synthesized protein is at least 80%.

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