Methods for producing recombinant proteins

Details Methods for producing recombinant proteins Methods for producing recombinant proteins

C - Chemistry, Metallurgy

07

K

C07K 1/14 (2006.01) C07K 14/81 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2384828

Methods are provided for producing and recovering recombinant proteins from plant tissue. The methods of the invention find use in the commercial processing of grains, particularly in the wet-milling of corn kernels. The methods involve steeping plant tissue and recovering the recombinant proteins in steep water. The methods additionally involve optimizing nucleic acid constructs and plants for the recovery of recombinant proteins from plant tissue.

L'invention concerne des procédés permettant de produire et de récupérer des protéines recombinantes à partir d'un tissu végétal. Les procédés décrits trouvent une application dans le traitement commercial de grains, particulièrement dans l'extraction de l'amidon des grains de maïs par voie humide. Ces procédés consistent à tremper le tissu végétal et à récupérer les protéines recombinantes dans l'eau de trempage. Les procédés consistent en outre à optimiser les produits de synthèse d'acide nucléique et les plantes en vue de la récupération de protéines recombinantes à partir d'un tissu végétal.

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