Methods for producing rna viruses from cdna

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/43 (2006.01) A61K 39/13 (2006.01) C07K 14/105 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 7/02 (2006.01) C12N 7/04 (2006.01) C12Q 1/70 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2067337

2067337 9203538 PCTABSCORE2 The invention relates to methods for producing RNA virus cDNA, methods for producing viable, RNA virus and viable, RNA virus produced by these methods. A novel RNA virus cDNA, recombinant DNA molecules containing that cDNA, and hosts transformed with those recombinant cDNA molecules are described. Novel methods for screening for variants of a strain 3 poliovirus and methods for increasing the attenuation of a strain poliovirus are described. This invention provides a vaccine useful for immunizing a subject against infectious poliovirus, wherein the vaccine comprises an effective amount of an RNA virus and a suitable carrier. A method of immunizing a subject against infectious poliovirus by administering the subject a suitable dose of the vaccine described hereinabove is described.

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