Methods for producing soluble multi-membrane-spanning proteins

Details Methods for producing soluble multi-membrane-spanning proteins Methods for producing soluble multi-membrane-spanning proteins

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/705 (2006.01) C07K 16/18 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2592390

Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.

La présente invention a trait à des procédés pour la production de protéines transmembranaires en rendements élevés, à conformation native, et/ou en forme soluble comprenant la solubilisation dans des détergents non ioniques ou zwitterrioniques ainsi que l'utilisation de promoteurs et de vecteurs d'expression pour l'expression à rendements élevés de polypeptides transmembranaires dans des cellules bactériennes. Des promoteurs mutés assurent une contrôle strict sur des polypeptides transmembranaires dans des hôtes de cellules bactériennes

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