Methods for profiling and classifying tissue using a...

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2389220

A sample of normal tissue specimens obtained from a subset of a population of subjects with shared characteristics is profiled in order to generate a plurality of structural indices that correspond to statistically significant representations of characteristics of tissue associated with the population. The structural indices include cell density, matrix density, blood vessel density and layer thickness. Alternatively, the sample of normal tissue specimens obtained from the subset of the population of subjects with shared characteristics can also be profiled in order to generate a plurality of cell function and/or mechanical indices that correspond to statistically significant representations of characteristics of tissue associated with the population. Structural, mechanical and/or cell function indices for a plurality of tissue populations are determined, and then stored in a database. The database information is then used to classify tissue specimens (e.g., human tissue specimens, animal tissue specimens, plant tissue specimens, food tissue specimens, or manufactured tissue specimens) provided by a user. In particular, a user measures parameters (e.g., structural, mechanical and/or cell function indices) associated with the user's tissue specimens and then compares this information to corresponding parameters for normal tissue in the database in order to classify the user's tissue specimens as either normal or abnormal.

On crée un profil à partir d'un échantillon de spécimens de tissus normaux obtenus à partir d'un sous-groupe d'une population de sujets partageant des caractéristiques en vue de générer plusieurs indices structurels qui correspondent aux représentations importantes sur le plan statistique de caractéristiques de tissus associés à la population. Les indices structurels comprennent la densité de la cellule, de la matrice, des vaisseaux sanguins et l'épaisseur de couche. En outre, on peut également créer un profil de l'échantillon de spécimens de tissus normaux obtenus à partir du sous-groupe de la population de sujets partageant des caractéristiques en vue de générer plusieurs indices mécaniques et/ou de fonction cellulaire qui correspondent aux représentations importantes sur le plan statistique des caractéristiques de tissus associées à la population. On détermine des indices structurels, mécaniques et/ou de fonctions cellulaires destinés à plusieurs populations de tissus, et on les stocke ensuite dans une base de données. On utilise alors les informations de la base de données pour classifier des spécimens de tissus (par ex., des spécimens de tissus humains, de tissus animaux, de tissus végétaux, de tissus alimentaires ou de tissus fabriqués) fournis par l'utilisateur. Notamment, un utilisateur mesure des paramètres (par exemple, des indices structurels, mécaniques et/ou de fonction cellulaire) liés aux spécimens de tissus de l'utilisateur et compare alors ces informations aux paramètres correspondants de tissus normaux de la base de données en vue de classer les spécimens de tissus d'utilisateur comme étant normaux ou anormaux.

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