Methods for protein analysis using protein capture arrays

G - Physics – 01 – N

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G01N 33/68 (2006.01) C07K 17/00 (2006.01)

Patent

CA 2470083

The invention relates to a method allowing identification and/or quantifying and/or characterizing proteins in a protein mixture, wherein the proteins are stratified on feature(s) on an array, and a procedure, preferably mass spectrometric analysis, is applied on the proteins on the feature(s), allowing determination of the nature and quantities of the proteins. In particular, the method allows the comparative analysis of nature and amount of proteins in at least two samples. It also allows the targeted selection of proteins out of a mixture of proteins. It further identifies three-dimensional structures that can interact with a selected target protein or a modification of said protein.

L'invention concerne une méthode permettant d'identifier et/ou de quantifier et/ou de caractériser des protéines dans un mélange de protéines, ces protéines étant superposées en fonction des caractéristiques sur un réseau, et un procédé, de préférence une analyse spectrométrique de masse, étant appliqué sur les protéines en fonction des caractéristiques, ce qui permet de déterminer la nature et la quantité des protéines. Plus particulièrement, cette méthode permet d'effectuer une analyse comparative de la nature et de la quantité de protéines dans au moins deux échantillons. Elle permet également la sélection ciblée de protéines extraites d'un mélange de protéines. Ladite méthode permet aussi d'identifier les structures tridimensionnelles qui interagissent avec une protéine cible sélectionnée ou une modification de ladite protéine.

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