Methods for providing internal references for use in...

Details Methods for providing internal references for use in... Methods for providing internal references for use in...

G - Physics

01

N

167/44

G01N 33/543 (2006.01)

Patent

CA 1286987

Abstract A method is disclosed for providing internal references for use in an analyte-receptor assay, for the determination of a target analyte in a sample. The assay uses a signal producing system and an analyte receptor capable of binding the target analyte at a discrete test zone on a solid phase, in an amount directly proportional to the amount of target analyte in the sample. The method comprises binding a reference receptor, selected to bind with an analyte receptor conjugate capable of complexation with the target analyte, at a discrete reference zone on a solid phase. The reference receptor is selected such that the rates of binding of the analyte receptor conjugate to the reference receptor and to the target analyte are direct- ly proportional to the amounts of reference receptor at the reference zone and target analyte bound at the test zone on the solid phase, and the rate constants are substantially equivalent.

532565

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