C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.12, 150/8.
C12N 15/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2011818
-0- METHODS FOR PURIFICATION, AMPLIFICATION AND DETECTION OF A NUCLEIC ACID Abstract of the Disclosure A targeted nucleic acid in a biological specimen can be purified and prepared for amplifica- tion or other uses by contacting the specimen with a purification reagent specific for that nucleic acid. This reagent comprises a nucleic acid fragment complementary to the targeted nucleic acid, which fragment is attached to nonporous, nonmagnetic particles. An insoluble hybrid is thus formed which is then separated from the specimen. After dehybridization, the purified targeted nucleic acid is available for further use, such as amplification by polymerase chain reaction and detection.
Burdick Brent A.
Chang Chu-An
Fyles Janet L.
Oakes Fred T.
Burdick Brent A.
Chang Chu-An
Chu-An Chang To Cetus Corporation
Fyles Janet L.
Gowling Lafleur Henderson Llp
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