Methods for purification of alpha-1-antitrypsin and...

C - Chemistry – Metallurgy – 07 – K

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C07K 1/14 (2006.01) C07K 1/16 (2006.01) C07K 1/30 (2006.01) C07K 1/36 (2006.01) C07K 14/775 (2006.01) C07K 14/81 (2006.01)

Patent

CA 2694854

This invention relates to protein separation and purification methods for both alpha- 1 -antitrypsin (AAT, also known as alpha-1 proteinase inhibitor, API, and A1-Pl) and Apolipoprotein A-I (ApoA- 1) from, for example, a fraction of human blood plasma. In certain embodiments, the invention provides methods for separating AAT from ApoA-1 at the initial stage of purification, so that the same starting material can be used as a source for both proteins. The methods further pertain to providing compositions of AAT and of ApoA-1 suitable for pharmaceutical use and are suitable for large-scale purification.

L'invention concerne des procédés de séparation et de purification de protéines appliqués à l'alpha-1-antitrypsine (AAT, également connue en tant qu'inhibiteur de protéinase alpha-1, API, et A1-Pl) et à l'apolipoprotéine A-I (ApoA-l) provenant par exemple d'une fraction de plasma sanguin humain. Certains modes de réalisation de l'invention concernent des procédés pour séparer l'AAT et l'ApoA-l lors de l'étape initiale de purification, de sorte que le même matériel de départ peut être utilisé en tant que source pour les deux protéines. Les procédés permettent en outre d'obtenir des compositions, contenant l'AAT et l'ApoA-l, qui sont appropriées pour un usage pharmaceutique ainsi que pour une purification à grande échelle.

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