Methods for purification of single-chain and double- chain...

Details Methods for purification of single-chain and double- chain... Methods for purification of single-chain and double- chain...

C - Chemistry, Metallurgy

12

N

195/39.1

C12N 9/68 (2006.01) C12N 9/72 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1284961

ABSTRACT OF THE DISCLOSURE A mixture containing a single-chain tissue plasminogen activator (tPA) and/or double-chain tPA is brought into close contact with a column carrying an immobilized Erythrina trypsin inhibitor as an affinity agent. Adsorbed proteins are eluted with eluents having different pHs with or without arginine or benzamidine, so that single-chain tPA is obtained in the eluent with a pH at least 4.5 and double-chain tPA is obtained in the eluent with a pH lower than 4.5.

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