Methods for purification of single domain antigen binding...

Details Methods for purification of single domain antigen binding... Methods for purification of single domain antigen binding...

C - Chemistry, Metallurgy

07

K

C07K 16/00 (2006.01) A61K 39/395 (2006.01) C07K 1/18 (2006.01) C07K 1/22 (2006.01) C07K 16/06 (2006.01) C07K 16/24 (2006.01)

Patent

CA 2739352

Processes and methods of purifying or separating single domain antigen binding (SDAB) molecules that include one or more single binding domains (e.g., one or more nanobody molecules), substantially devoid of a complementary antibody domain and an immunoglobulin constant region, using Protein A-based affinity chromatography, are disclosed.

La présente invention concerne des procédures et procédés de purification ou séparation de molécules de liaison dantigène monodomaines (SDAB) qui comprennent un ou plusieurs domaines de liaison uniques (par exemple, une ou plusieurs molécules de nanocorps), sensiblement exemptes dun domaine danticorps complémentaire et dune région constante dimmunoglobuline, en utilisant la chromatographie daffinité basée sur la Protéine A.

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