Methods for purified aav vector production

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/86 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12N 15/861 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2319212

The invention is directed to novel systems for the high level production of purified recombinant adeno-associated virus (rAAV) vector stocks comprising producer cell lines and helper adenoviruses. These systems provide high level production of rAAV vector stocks that are not contaminated by helper viruses or have very minimal contamination with helper virus. The invention is also directed to methods for the production of high yield, purified rAAV vector stocks using the systems of the invention.

L'invention concerne de nouveaux systèmes permettant de produire dans des quantités élevées des réserves de vecteurs purifiés des virus recombinants associées aux adénovirus (rAAV), y compris les lignées cellulaires de production et les adénovirus auxiliaires. Ces systèmes permettent d'atteindre des niveaux élevés de production de réserves de vecteurs purifiés des rAAV qui ne sont pas contaminés par des virus auxiliaires ou dont la contamination par des virus auxiliaires est extrêmement limitée. L'invention concerne également des procédés de production de réserves à haut rendement de vecteurs purifiés des rAAV au moyen des systèmes de l'invention.

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