Methods for purifying non-chromosomal nucleic acid molecules...

Details Methods for purifying non-chromosomal nucleic acid molecules... Methods for purifying non-chromosomal nucleic acid molecules...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2340153

Methods are provided for purifying non-chromosomal nucleic acid molecules from cells, comprising: the general steps of (a) lysing cells to form a nucleic acid-containing lysate, and (b) applying the lysate to a depth filter in order to obtain a clarified solution containing purified non-chromosomal nucleic acid molecules.

L'invention concerne des procédés de purification de molécules d'acides nucléiques nonchromosomiques provenant de cellules. Les étapes principales consistent à lyser des cellules de façon à former un lysat contenant des acides nucléiques et à déposer le lysat sur un filtre de profondeur dans le but d'obtenir une solution clarifiée contenant des molécules d'acides nucléiques nonchromosomiques purifiées.

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