Methods for purifying nucleic acids

Details Methods for purifying nucleic acids Methods for purifying nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2338397

Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.

L'invention concerne des procédés relatifs à l'élaboration de compositions hautement purifiées à base d'acides nucléiques, qui consistent à engager un processus largement rationalisé et aisément automatisé. Lesdits procédés font appel à des mélangeurs statiques pour réaliser la lyse cellulaire et la précipitation de débris. Viennent ensuite une centrifugation et une chromatographie par échange d'ions. On peut inclure une étape de purification en utilisant l'ultrafiltration tangentielle. L'invention concerne également un processus évolutif permettant d'élaborer de l'ADN plasmidique de qualité pharmaceutique, utile en thérapie génique.

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