Methods for purifying nucleic acids

Details Methods for purifying nucleic acids Methods for purifying nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07H 1/06 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2261150

Methods are provided for producing highly purified compositions of nucleic acids by using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided, which is efficient and avoids the use of toxic organic chemicals.

On décrit des procédés de production de compositions très purifiées d'acides nucléiques, à l'aide d'une ultrafiltration tangentielle. On décrit également un procédé à l'échelle adaptable de production d'ADN plasmide de qualité pharmaceutique, utile en thérapie génique, ce procédé étant efficace et permettant d'éviter l'utilisation d'agents chimiques organiques toxiques.

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