Methods for purifying urokinase preparations

Details Methods for purifying urokinase preparations Methods for purifying urokinase preparations

C - Chemistry, Metallurgy

12

P

195/127, 195/81,

C12P 21/00 (2006.01)

Patent

CA 1040562

ABSTRACT OF THE DISCLOSURE A method of purifying a urokinase solution mixed with foreign proteins and pyrogenic substances, in which the aqueous urokinase solution is partially saturat?d with an agent which precipitates proteins to an extent, depending on the initial protein concentration of the solution, such that the partial sa- turation precipitates only part of the proteins in the initial solution, the part being sufficiently small for its urokinase content to be kept within predetermined proportions which are small or practically negligible, and the supernatant liquid containing the urokinase is collected. The purified urok- nase so obtained can be converted in to urokinase hepavinate, which is useful in the treatment of thrombo-embolic syndromes.

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