Methods for quantification of micrornas and small...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2562390

The invention relates to ribonucleic acids, probes and methods for detection, quantification as well as monitoring the expression of mature microRNAs and small interfering RNAs (siRNAs). The invention furthermore relates to methods for monitoring the expression of other non-coding RNAs, mRNA splice variants, as well as detecting and quantifying RNA editing, allelic variants of single transcripts, mutations, deletions, or duplications of particular exons in transcripts, e.g., alterations associated with human disease such as cancer. The invention furthermore relates to methods for detection, quantification as well as monitoring the expression of deoxy nucleic acids.

L'invention concerne des acides ribonucléiques, des sondes et des méthodes permettant de détecter, de quantifier et de surveiller l'expression de micro-ARN matures et de petits ARN interférants (siARN). L'invention concerne également des méthodes permettant de surveiller l'expression d'autres ARN non codants et de variantes d'épissure d'ARNm, de détecter et de quantifier une édition d'ARN, des variantes alléliques de transcriptions uniques, des mutations, des suppressions ou des duplications d'exons particuliers dans des transcriptions, par exemple, des altérations associées à une maladie humaine telle que le cancer. L'invention concerne enfin des méthodes permettant de détecter, de quantifier et de surveiller l'expression d'acides déoxy ribo nucléiques.

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