Methods for quantitative proteome analysis of glycoproteins

Details Methods for quantitative proteome analysis of glycoproteins Methods for quantitative proteome analysis of glycoproteins

G - Physics

01

N

G01N 33/68 (2006.01) C12Q 1/34 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2487314

The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.

L'invention concerne un procédé d'identification et de quantification de polyglycopeptides dans un échantillon. Le procédé consiste à immobiliser des glycopolypeptides sur un support solide, à cliver les glycopolypeptides immobilisés, libérant ainsi es peptides non glycosilés et retenant les glycopeptides immobilisés, à libérer les glycopeptides du support solide, et à analyser les glycopeptides libérés. Le procédé peut ensuite consister à identifier un ou plusieurs glycopeptides, par exemple, à l'aide de la spectrométrie de masse.

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