Methods for rapid cloning of full length cdnas

Details Methods for rapid cloning of full length cdnas Methods for rapid cloning of full length cdnas

C - Chemistry, Metallurgy

07

K

C07K 5/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2288988

The present invention provides a method for high throughput cloning of full length cDNA sequences. This method uses a plurality of clone arrays prepared from cDNA libraries which have been preferably enriched for 5' mRNA sequences and size fractionated into several discrete ranges (sub-libraries). These arrays are used to rapidly identify the full length cDNA sequence for a DNA segment of interest.

La présente invention concerne une méthode permettant le clonage à haut rendement de séquences d'ADNc de longueur totale. Ladite méthode utilise une pluralité d'ensembles de clones préparés à partir de banques d'ADNc, qui ont été de préférence enrichies en séquences d'ARNm 5' et fractionnées selon leur taille en plusieurs gammes distinctes (sous-banques de clones). Ces ensembles sont utilisés pour identifier rapidement la séquence d'ADNc de longueur totale correspondant à un segment d'ADN d'intérêt.

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