Methods for rapidly transforming monocots

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01)

Patent

CA 2661825

The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

La présente invention propose des procédés pour transformer des plantes monocotylédones par le biais d'un protocole simple et rapide permettant d'obtenir des plantes régénérées pouvant être plantées après 4 à 8 semaines seulement. Un milieu de culture cellulaire et des conditions de croissance associés sont également proposés, ainsi que des plantes et des parties de plantes obtenues au moyen du procédé. De plus, un procédé permettant de cribler des génotypes végétaux récalcitrants à une transformation par les procédés de la présente invention est également proposé. En outre, un système visant à agrandir une fenêtre de développement par priorité afin de produire des plantes transgéniques au moyen des procédés de la présente invention est également fourni.

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