C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) C07K 16/10 (2006.01) C12N 15/13 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2695309
The invention relates to a method for manufacturing recombinant anti-RSV antibodies and antibody compositions. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one distinct anti-RSV antibody. The cells are cultured under suitable conditions for expression of the anti-RSV antibody/antibodies. The nucleic acid sequence is introduced into the cells by transfection with expression vectors, which avoid site-specific integration. The present method is suitable for manufacturing recombinant mono- and polyclonal anti-RSV antibodies for therapeutic uses. The invention relates to a method for manufacturing recombinant anti-RSV antibodies and antibody compositions. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one distinct anti-RSV antibody. The cells are cultured under suitable conditions for expression of the anti-RSV antibody/antibodies. The nucleic acid sequence is introduced into the cells by transfection with expression vectors, which avoid site-specific integration. The present method is suitable for manufacturing recombinant mono-- and polyclonal anti-RSV antibodies for therapeutic uses.
Cette invention concerne une méthode de production recombinée d'anticorps anti-RSV et de compositions d'anticorps. La méthode consiste à obtenir un ensemble de cellules transfectées avec un ensemble de séquences d'acides nucléiques variantes, chaque cellule de l'ensemble étant transfectée avec un anticorps anti-RSV distinct et pouvant exprimer celui-ci. Les cellules sont cultivées dans des conditions appropriées pour l'expression de l'anticorps ou des anticorps anti-RSV. La séquence d'acides nucléiques est introduite dans les cellules par transfection avec des vecteurs d'expression, ce qui empêche une intégration propre au site. La méthode de l'invention permet de produire de manière appropriée des anticorps anti-RSV monoclonaux et polyclonaux recombinants à des fins thérapeutiques.
Lantto Johan
Nielsen Lars Soegaard
Tolstrup Anne Bondgaard
Wiberg Finn
Sim & Mcburney
Symphogen A/s
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