C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/00 (2006.01) C07K 1/22 (2006.01) C07K 16/06 (2006.01) C07K 16/40 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12N 11/00 (2006.01) G01N 33/531 (2006.01)
Patent
CA 2224687
In accordance with the present invention, there are provided novel methods and reagents for reducing background binding in antibody preparations having an unwanted affinity for intracellular protein(s). The invention method comprises treating an antibody preparation with permeabilized cells, then separating the antibody preparation from the permeabilized cells. The reagents are useful for reducing the level of background binding in antibody preparations. Also provided are antibody preparations that are substantially free of background binding.
Cette invention concerne des méthodes et des réactifs nouveaux qui permettent de diminuer la fixation de fond dans les préparations d'anticorps possèdant une affinité indésirable pour une ou plusieurs protéines intracellulaires. La méthode consiste à traiter la préparation d'anticorps par des cellules perméabilisées, puis à séparer la préparation d'anticorps de ces cellules. Les réactifs permettent de réduire le niveau de fixation du fond dans les préparations d'anticorps. L'invention concerne également des préparations d'anticorps sensiblement dépourvues de fixation de fond.
Cushing Susan
Vidrich Alda
Cedars-Sinai Medical Center
Gowling Lafleur Henderson Llp
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