Methods for reducing complexity of nucleic acid samples

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2419613

The invention provides several methods for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids on a nucleic acid probe array. The methods result in a subset of the initial population enriched for a desired property, or lacking nucleic acids having an undesired property. The resulting nucleic acids in the subset are then applied tothe array for various types of analysis. The methods are particularly useful for analyzing populations having a high degree of complexity, for example, chromosomal-derived DNA, or whole genomic DNA, or mRNA population. In addition, such methods allow for analysis of pooled samples.

La présente invention concerne plusieurs méthodes permettant de réduire la complexité d'une population d'acides nucléiques avant le démarrage de l'analyse des acides nucléiques sur une matrice de sondes d'acides nucléiques. Ces méthodes permettent d'obtenir un sous-ensemble de la population de départ enrichi de manière à présenter une propriété souhaitée ou ne contenant pas d'acides nucléiques présentant une propriété non souhaitée. Les acides nucléiques ainsi obtenus dans le sous-ensemble sont ensuite appliqués sur la matrice pour différents types d'analyses. Ces méthodes sont particulièrement utiles pour analyser des populations présentant un degré élevé de complexité, par exemple, un ADN chromosomal, un ADN génomique entier, ou une population d'ARNm. En outre, ces méthodes permettent d'analyser des échantillons regroupés.

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