Methods for reducing enzyme consumption in second generation...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/14 (2006.01) C12P 19/00 (2006.01) D21C 3/00 (2006.01) D21C 5/00 (2006.01)

Patent

CA 2713837

Provided are methods of liquefaction, saccharification and fermentation of pre- treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter ( > 20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading < 7 FPU (g DM)-1. At high dry matter content, cellulase enzyme consumption can be reduced, without loss of hydrolysis yield, by addition of small quantities of PEG or surfactant.

L'invention se rapporte à des procédés de liquéfaction, de saccharification et de fermentation de biomasse lignocellulosique préalablement traitée entrant dans la production de bioéthanol. L'addition de PEG ou d'un tensioactif est efficace pour augmenter les rendements de l'hydrolyse par cellulase avec une grande quantité de matière sèche (> 20 %) et à des concentrations étonnamment basses par rapport aux résultats obtenus en hydrolysant une faible quantité de matière sèche. Cet effet est le plus visible avec un chargement de cellulase comparativement faible inférieur à 7 UPF (g DM)-1. Avec une grande quantité de matière sèche, on peut réduire la consommation d'enzymes cellulase sans pertes en termes de rendement hydrolytique, et ce en ajoutant de petites quantités de PEG ou de tensioactif.

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