Methods for reducing non-specific binding to a nucleic acid...

Details Methods for reducing non-specific binding to a nucleic acid... Methods for reducing non-specific binding to a nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2326498

The present invention provides a variety of methods for reducing non-specific binding of a target molecule or plurality of target molecules to an array of oligonucleotides. The methods of the present invention include surface modification techniques and oligonucleotide modification techniques. According to one method of the present invention, non-specific binding of a target molecule to an array of oligonucleotides is reduced by replacing at least one of: i) the protecting groups on each of the plurality of oligonucleotides, and ii) the protecting groups on each of the protected regions of the substrate, with a negatively charged phosphate residue.

La présente invention concerne divers procédés permettant de réduire les liaisons non spécifiques d'une molécule cible ou d'une pluralité de molécules cibles avec une matrice d'oligonucléotides. On a recours à cet effet à des techniques de modification de surface ainsi qu'à des techniques de modification des oligonucléotides. L'un des procédés de l'invention consiste à réduire la liaison non spécifique d'une molécule cible avec une matrice d'oligonucléotides en remplaçant par un groupe fonctionnel phosphate négativement chargé les uns au moins des groupes parmi les suivants: (i) les groupes de protection présents sur chacun des oligonucléotides de la pluralité d'oligonucléotides, et (ii) les groupes de protection présents sur chacune des régions protégées du substrat.

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