Methods for reducing the formation of byproducts in the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/16 (2006.01) C07K 14/58 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2379100

A method for reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium in a method for producing a polypeptide containing a serine residue by culturing transformed cells, and a method for producing a polypeptide containing a serine residue by culturing transformed cells, characterized by reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium.

L'invention concerne un procédé mis en oeuvre dans un procédé de production par culture de cellules transformées d'un polypeptide possédant un groupe sérine, à savoir un procédé permettant d'inhiber la formation d'un produit secondaire polypeptidique dans lequel est incorporé un groupe 0-acétylsérine, par addition au milieu de culture d'au moins un élément choisi dans le groupe comprenant l'histidine, la méthionine et la glycine; et un procédé de production par culture de cellules transformées d'un polypeptide possédant un groupe sérine, caractérisé en ce que la formation d'un produit secondaire polypeptidique dans lequel est incorporé un groupe 0-acétylsérine est inhibée par addition au milieu de culture d'au moins un élément choisi dans le groupe comprenant l'histidine, la méthionine et la glycine.

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