C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/113 (2006.01) C07K 14/505 (2006.01) C07K 14/515 (2006.01) C07K 14/535 (2006.01) C07K 14/56 (2006.01) C07K 14/61 (2006.01) C07K 14/78 (2006.01)
Patent
CA 2408851
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
La présente invention concerne des nouveaux procédés de préparation et de repliement de protéines insolubles ou agrégées renfermant des cystéines libres, une cellule hôte exprimant la protéine étant exposée à un agent bloquant la cystéine. On peut modifier ensuite les protéines solubles et repliées obtenues au moyen des nouveaux procédés, afin d'accroître leur efficacité. Ces modifications consistent à fixer un groupe PEG en vue de former des protéines PEGylées.
Cox George N.
Doherty Daniel H.
Rosendahl Mary S.
Bolder Biotechnology Inc.
Gowling Lafleur Henderson Llp
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