Methods for regulating transcription by targeting quadruplex...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 31/427 (2006.01) A61P 35/00 (2006.01) C07D 487/22 (2006.01) C07D 513/22 (2006.01)

Patent

CA 2496303

Among the different intrastrand quadruplex structures that can arise from duplex DNA, it has been discovered that the nucleotide sequences (GGA)4 and (GGA)3GG form biologically significant quadruplex structures. Thus, provided herein are methods for identifying molecules that modulate the biological activity of quadruplex DNA comprising the nucleotide sequence (GGA)4 or the nucleotide sequence (GGA)3GG, and specifically, methods for identifying molecules that bind such quadruplexes. Also provided herein are methods for modulating the biological activity of a biologically significant native quadruplex DNA with a molecule identified by the methods described herein.

Parmi les structures quadruplex intrabrin qui peuvent survenir à partir d'ADN duplex, on a découvert que les séquences nucléotidiques (GGA)4 et (GGA)3GG forment des structures quadruplex biologiquement significatives. La présente invention concerne donc des techniques d'identification de molécules qui modulent l'activité biologique d'ADN quadruplex comprenant la séquence nucléotidique (GGA)4 ou la séquence nucléotidique GGA)3GG, et plus particulièrement, des techniques d'identification de molécules qui se lient à ces quadruplexes. Cette invention concerne aussi des techniques de modulation de l'activité biologique d'ADN quadruplex natif biologiquement significatif avec une molécule identifiée par les techniques de cette invention.

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