C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/18 (2006.01) C07K 1/34 (2006.01) C07K 1/36 (2006.01)
Patent
CA 2731943
Methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant are described, which methods comprise the sequential steps of: (a) passing the composition through an ion exchange membrane. where the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and the at least one contaminant, and (b) recovering the purified polypeptide from the effluent.
La présente invention concerne des procédés de purification d'un polypeptide à partir d'une composition comprenant le polypeptide et au moins un contaminant, les procédés comprenant la suite détapes consistant à : (a) faire passer la composition à travers une membrane échangeuse d'ions, le polypeptide et la membrane ayant des charges opposées, dans les conditions de fonctionnement comprenant un tampon ayant un pH suffisamment distinct du pI du polypeptide, afin d'augmenter la charge du polypeptide, et une faible force ionique efficace pour empêcher le blindage des charges par les ions du tampon, ce qui entraîne la liaison de la membrane au polypeptide et au(x) contaminant(s), et (b) récupérer le polypeptide purifié à partir de l'effluent.
Bill Jerome Jr.
Brown Arick
Dowd Christopher
Tully Timothy
Genentech Inc.
Smart & Biggar
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