Methods for screening for gene specific hybridization...

Details Methods for screening for gene specific hybridization... Methods for screening for gene specific hybridization...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2611788

A method for identification of gene specific hybridization polymorphisms (GSHPs) and their use is presented. The method involves the steps of a) global screening for hybridization polymorphisms using microarray; b) enzyme mediated genome complexity reduction; c) enzyme mediated differential signal amplification and noise reduction; d) data extraction and GSHP identification; and e) use of GSHPs in high throughput screening.

La présente invention concerne un procédé d~identification de polymorphismes d~hybridation spécifiques de gène (GSHPs) et leur utilisation. Le procédé comprend les étapes suivantes : a) criblage global de polymorphismes d'hybridation à l~aide d~un microréseau ; b) réduction de la complexité du génome induite par une enzyme ; c) réduction du bruit et amplification du signal différentiel induites par une enzyme ; d) extraction de données et identification GSHP ; et e) utilisation de GSHP pour un criblage à débit élevé.

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