Methods for screening nuclear transcription factors for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2296015

This invention provides methods of screening a nuclear transcription factor ligand for the ability to modulate estrogen activation at an AP-1 site. The methods involve the steps of: a) providing a first cell containing an estrogen receptor, a receptor for the nuclear transcription factor ligand, and a promoter comprising an AP-1 site which regulates expression of a first reporter gene; b) contacting the first cell with the transcription factor ligand and with a compound having AP-1 mediated estrogenic activity; and c) detecting expression of the first reporter gene.

Cette invention concerne des procédés de criblage d'un ligand du facteur de transcription nucléaire en fonction de son aptitude à moduler l'activation des oestrogènes au niveau d'un site AP-1. Le procédé comprend les étapes suivantes: a) on utilise une première cellule contenant un récepteur des oestrogènes, un récepteur pour le ligand du facteur de transcription nucléaire et un promoteur comprenant un site AP-1 qui régule l'expression d'un premier gène marqueur; b) on met en contact la première cellule avec le ligand du facteur de transcription et avec un composé ayant une activité oestrogénique induite par AP-1; et c) on détecte l'expression du premier gène marqueur.

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