Methods for screening substances in a microwell array

Details Methods for screening substances in a microwell array Methods for screening substances in a microwell array

B - Operations, Transporting

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B01L 3/00 (2006.01) B01L 99/00 (2010.01) B01L 3/02 (2006.01) C40B 60/00 (2006.01) G01N 35/00 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2446157

Methods for manufacturing and using an apparatus for manipulating and analyzing a large number of microscopic samples of a liquid or materials, including cells, in liquid suspension. Parallel through-holes (12) are formed in a platen (10) and loaded with a liquid. Loading may be performed in such a way as to create a gradient, with respect to the position of the through- holes, of the concentration of a particular substance or of another quantity. Mixing of the contents of through-holes may be obtained by bringing filled microwell arrays into contact with each other with registration of individuals through-holes.

L'invention porte sur des procédés de fabrication et d'utilisation d'un appareil de manipulation et d'analyse d'un grand nombre d'échantillons microscopiques de matériaux liquides dont des cellules en suspension dans des liquides. Des trous traversants parallèles formés dans une platine sont remplis de liquide. Le remplissage peut se faire de manière à créer un gradient fonction de la position des trous, de la concentration d'une substance particulière ou d'une autre entité. Le mélange des contenus des trous peut se faire en mettant en contact les réseaux de micro-puits entre eux et en coïncidence avec des trous individuels

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