Methods for selecting germ-line competent cells in chicken...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/06 (2006.01) A01K 67/027 (2006.01)

Patent

CA 2282791

Methods are described for selecting germ-line competent cells in stage X (E-G & K) chicken embryos comprising selecting cells from a stage X (E-G & K) chicken embryo which have an epitope expressed by germ-line competent cells associated with their cell surface. The epitope may be EMA-1 or SSEA-1, for example. Methods for producing a chimeric chicken embryo and a chimeric chicken using the selected germ-line competent cells are also described.

On décrit des procédés de sélection de cellules compétentes de lignées germinales dans des embryons de poulets en phase X(E-G & K); ces procédés consistent à sélectionner des cellules dans des embryons de poulets en phase X(E-G & K) qui ont un épitopes exprimé par des cellules compétentes de lignées germinales associées à leur surface cellulaire. L'épitope peut être EMA-1 ou SSEA-1, par exemple. On décrit également des procédés de production d'un embryon de poulet chimère et d'un poulet chimère dans lesquels on utilise les cellules compétentes de lignées germinales sélectionnées.

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