Methods for selecting improved strains

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/01 (2006.01) C12N 1/14 (2006.01)

Patent

CA 2674715

The present invention relates to a system for improving a strain of filamentous fungus that is useful in producing an enzyme of interest such as a cellulase. The invention involves generating genetic diversity in a strain of fungal cells, subjecting the population of cells to selection, and isolating the cells that overproduce the enzyme. The selection techniques involve culturing the population of cells in purified cellulose and/or at an elevated temperature that is adverse to production of the enzyme.

La présente invention concerne un système permettant d'améliorer une souche de champignon filamenteux utilisée dans la production d'une enzyme d'intérêt, telle qu'une cellulase. Cette invention consiste à générer une diversité génétique dans une souche de cellules fongiques, à soumettre la population de cellules à une sélection et à isoler les cellules qui surproduisent l'enzyme. Les techniques de sélection impliquent la mise en culture de la population de cellules dans une cellulose purifiée et/ou à une température élevée qui va à l'encontre de la production de l'enzyme.

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