C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) G01N 33/50 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2122141
Methods are provide for increasing the number of human hematopoietic precursor cells in vitro, comprising the general steps of (a) separating CD34-positive human hematopoietic precursor cells from CD34-negative cells present in a blood product; (b) inoculating the separated precursor cells at an initial density of between 1×10 3 cells/ml and 4× 10 6 cells/ml into a culture vessel containing a culture medium comprising a nutritive medium and hematopoietic growth factors effective for expansion of CD34-positive cells, with the proviso that said culture medium and culture vessel contain no stromal cells or stromal cell conditioned medium; and (c) culturing the precursor cells under conditions and for a time sufficient to increase the number of precursor cells relative to the number of precursor cells present after separation of hematopoietic cells from said blood product.
Berenson Ronald J.
Fei Ruigao
Goffe Randal A.
Heimfeld Shelly
Peterson Dale R.
Cellpro Incorporated
Gowling Lafleur Henderson Llp
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