Methods for selectively stimulating proliferation of t cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/08 (2006.01) C07K 7/04 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 5/06 (2006.01) C12N 5/16 (2006.01)

Patent

CA 2191586

Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population of T cells, an accessory molecule on the surface of the T cells, such as CD9 or CD28, is stimulated with a ligand (e.g. monoclonal antibody ES5.2D8) specific for CD9 which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

Procédé entraînant la prolifération d'une population de cellules T par activation de ladite population et stimulation d'une molécule accessoire présente à la surface desdites cellules T par un ligand qui se fixe à ladite molécule. La prolifération des cellules T se produit alors en l'absence de facteur de croissance exogène ou de cellules accessoires. L'activation des cellules T résulte de la stimulation du complexe récepteur de la cellule T (TCR)/CD3 ou de la protéine de surface CD2. Pour entraîner la prolifération d'une population de cellules T activées, on stimule une cellule accessoire à la surface des cellules T telles que les CD9 et CD28 par un ligand (p. ex. l'anticorps monoclonal ES5.2D8) spécifique du CD9 qui se fixe à la molécule accessoire. La population de cellules T amplifiée par ce procédé peut servir à des transductions génétiques, à l'immunothérapie et à des diagnostics.

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