Methods for separation, characterization, and/or...

G - Physics – 01 – N

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G01N 21/65 (2006.01) C12M 1/12 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2741036

The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.

La présente invention porte sur un procédé de séparation, de caractérisation et/ou d'identification de microorganismes dans un échantillon danalyse. Le procédé selon l'invention comporte une étape facultative de lyse pour la lyse de cellules qui ne sont pas des microorganismes qui peuvent être présentes dans l'échantillon danalyse, suivie par une opération de séparation ultérieure. Le procédé peut être utile pour la séparation, la caractérisation et/ou l'identification de microorganismes à partir d'échantillons complexes tels qu'un milieu de culture contenant du sang. L'invention porte en outre sur une interrogation spectroscopique de Raman de l'échantillon de microorganisme séparé afin de produire des mesures du microorganisme et de caractériser et/ou didentifier le microorganisme dans l'échantillon à l'aide desdites mesures spectroscopiques de Raman.

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