Methods for separation, characterization and/or...

G - Physics – 01 – N

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G01N 21/65 (2006.01) C12M 1/12 (2006.01) C12M 1/34 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2740836

The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

La présente invention porte sur un procédé pour la séparation, la caractérisation et/ou l'identification de microorganismes dans un échantillon test. Le procédé selon l'invention comprend une étape de lyse facultative pour la lyse de cellules qui ne sont pas des microorganismes, les cellules pouvant être présentes dans un échantillon test, suivie d'une étape de séparation ultérieure. Le procédé peut être utile pour la séparation, la caractérisation et/ou l'identification de microorganismes provenant d'échantillons complexes tels qu'un milieu de culture contenant du sang. L'invention prévoit en outre une interrogation spectroscopique de l'échantillon de microorganisme séparé pour produire des mesures du microorganisme et caractériser et/ou identifier le microorganisme dans l'échantillon à l'aide desdites mesures spectroscopiques.

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