Methods for simultaneously detecting both members of a...

Details Methods for simultaneously detecting both members of a... Methods for simultaneously detecting both members of a...

G - Physics

01

N

G01N 33/543 (2006.01)

Patent

CA 2380487

Simultaneous immunological determination of both members A and B of a binding pair (e.g. antigen and antibody) in a biological sample, by: 1) incubating the sample with anti-A antibody attached to particles, to produce particle-antibody-A complex; 2) incubating the complex with labelled anti-A and anti-B antibodies; and 3) detecting labels by flow cytometry.

L'invention concerne un procédé permettant l'analyse immunologique simultanée des deux parties A et B d'une paire de liaison (p. ex. antigène et anticorps) dans un échantillon biologique, au moyen des étapes suivantes : 1) incubation de l'échantillon avec un anticorps anti-A fixé sur des particules afin de produire un complexe particules anticorps-A ; 2) incubation du complexe avec des anticorps marqués anti-A et anti-B ; et 3) détection des marqueurs par cytométrie de flux.

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