C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/75 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2513227
The present invention provides methods and compositions for the construction and direct transformation of site-saturation libraries into Bacillus. This method avoids the need for the use of intermediate hosts, such as E. coli for the development of Bacillus strains suitable for the production of proteins.
L'invention concerne des procédés et des compositions destinés à la construction et à la transformation en Bacillus de banques formées par saturation de sites. Ce procédé permet d'éviter l'utilisation d'hôtes intermédiaires tels que E.coli pour la mise au point de souches Bacillus convenant pour la production de protéines.
Leeflang Chris
Van Der Kleij Wilhelmus A. H.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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