Methods for sterile aspiration/reinjection of bodily fluid

Details Methods for sterile aspiration/reinjection of bodily fluid Methods for sterile aspiration/reinjection of bodily fluid

A - Human Necessities

61

M

A61M 31/00 (2006.01) A61M 5/145 (2006.01) A61M 25/00 (2006.01) A61B 19/00 (2006.01)

Patent

CA 2454110

Methods for aspiration and filtering (95) of a bodily fluid containing undesired components are provided and for treatment of the bodily fluids in a sterile environment (100) in preparation for reinjection of treated aspirate into a donor subject. The invention methods are particularly designed to facilitate transfection of aspirated cells with angiogenesis promoting molecules without danger to the technician or the donor prior to reinjection of the treated cells, either percutaneously or via a surgical opening via catheter or injection needle (72).

L'invention se rapporte à des méthodes d'aspiration et de filtrage (95) de liquides organiques contenant des composants indésirables, ainsi qu'à des méthodes permettant de traiter ces liquides organiques dans un environnement stérile (100), en vue de réinjecter le prélèvement par aspiration traité dans un sujet donneur. Les méthodes selon l'invention sont plus particulièrement conçues pour faciliter la transfection de cellules aspirées au moyen de molécules favorisant l'angiogenèse, sans présenter de danger pour le manipulateur ou le donneur, préalablement à la réinjection des cellules traitées, soit de manière percutanée, soit par l'intermédiaire d'une ouverture chirurgicale au moyen d'un cathéter ou d'une aiguille d'injection (72).

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