Methods for storing conifer somatic embryo germinants

Details Methods for storing conifer somatic embryo germinants Methods for storing conifer somatic embryo germinants

A - Human Necessities

01

H

A01H 4/00 (2006.01) A01H 7/00 (2006.01) A01N 3/00 (2006.01) C12N 5/02 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2568476

In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5°C to 10°C for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24°C prior to transplantation into growth medium.

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