Methods for stratification and storage of somatic embryos

A - Human Necessities – 01 – H

Patent

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A01H 4/00 (2006.01)

Patent

CA 2698134

In one aspect, a method is provided for producing stratified fleet cotytedonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 niM/Kg lo 450 rriM/Kg at a temperature of from 22°C to 25°C for a firs! incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity: and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0°C to 10°C for a second incubation period of at least one week lo produce stratified cotyledonary somatic embryos.

Dans un aspect, l'invention concerne un procédé de production d'embryons somatiques de conifères cotylédonaires en parc stratifiés. Le procédé comprend (a) l'incubation d'une culture comprenant des embryons somatiques de conifère immature dans un récipient de culture comprenant un milieu de développement ayant une osmolalité de l'ordre de 300 niM/Kg à 450 rriM/Kg à une température de 22°C à 25°C pendant une première durée d'incubation de longueur suffisante pour qu'au moins une partie des embryons atteigne la maturité anatomique; et (b) la soumission des embryons dans le récipient de culture selon l'étape (a) à une température de 0°C à 10°C pendant une seconde période d'incubation d'au moins une semaine pour produire des embryons somatiques cotylédonaires stratifiés.

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