Methods for targeting, enriching, detecting and/or isolating...

Details Methods for targeting, enriching, detecting and/or isolating... Methods for targeting, enriching, detecting and/or isolating...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2263815

A method for targeting, enriching, detecting and isolating a double-stranded nucleic acid target sequence by adding heterologous probes to the reaction system along with the reduced amount of homologous probes in the process of homologous pairing and/or strand exchange between the homologous probe and the target sequence in the presence of a RecA-like recombinase.

Procédé pour cibler, enrichir, détecter et/ou isoler des séquences d'acides nucléiques cibles bicaténaires, ledit procédé consistant à ajouter des sondes hétérologues au système de réaction, avec un nombre réduit de sondes hétérologues dans le procédé d'appariement homologue et/ou l'échange de brins entre la sonde homologue et la séquence cible, en présence d'une recombinase de type RecA.

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