Methods for the collection and maturation of oocytes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/075 (2010.01) C12N 5/02 (2006.01)

Patent

CA 2761535

The present invention relates to a method of producing an embryo from an oocyte by an assisted reproduction technology. The method includes (a) collecting an oocyte from an ovary of a subject in a collection medium comprising a first phosphodiesterase inhibitor and an agent that increases intracellular cAMP concentration in the oocyte, (b) culturing the oocyte in a maturation medium comprising a second phosphodiesterase inhibitor, and (c) producing an embryo from the oocyte by an assisted reproduction technology. The present invention also relates to methods of inducing oocyte maturation. For example a method of in vitro maturation of an oocyte is described which comprises steps (a) and (b) above. The present invention also relates to an oocyte maturation medium comprising a phosphodiesterase inhibitor and a ligand for inducing maturation of the oocyte. A combination product comprising an oocyte collection and maturation medium referred to above is also described.

Cette invention concerne un procédé de production d'un embryon à partir d'un ovocyte par une technique de reproduction assistée. Le procédé comprend (a) le prélèvement d'un ovocyte à partir d'un ovaire d'un sujet dans un milieu de prélèvement comprenant un premier inhibiteur de phosphodiestérase et un agent qui accroît la concentration d'AMPc intracellulaire dans l'ovocyte, (b) la culture de l'ovocyte dans un milieu de maturation comprenant un second inhibiteur de phosphodiestérase, et (c) la production d'un embryon à partir de l'ovocyte par une technique de reproduction assistée. Cette invention concerne également des procédés permettant d'induire la maturation de l'ovocyte. Par exemple, on décrit un procédé de maturation in vitro d'un ovocyte qui comprend les étapes (a) et (b) ci-dessus. Cette invention concerne également un milieu de maturation d'ovocyte comprenant un inhibiteur de phosphodiestérase et un ligand pour induire la maturation de l'ovocyte. Un produit combiné comprenant un milieu de prélèvement et de maturation d'ovocyte mentionné ci-dessus est également décrit.

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