Methods for the detection, analysis and isolation of nascent...

Details Methods for the detection, analysis and isolation of nascent... Methods for the detection, analysis and isolation of nascent...

C - Chemistry, Metallurgy

07

H

C07H 21/02 (2006.01) C07H 1/00 (2006.01) C07K 1/13 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2383554

This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins trans-lated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently de-tected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s- indacene) dyes.

Cette invention a trait à des marqueurs non radioactifs facilitant la détection et l'analyse de nouvelles protéines transduites dans des systèmes de transduction, cellulaires ou dépourvus de cellule. Il est possible de détecter, d'isoler et d'analyser, facilement et rapidement, ces nouvelles protéines contenant ces marqueurs et ce, sans être confronté aux problèmes de manipulation et de mise au rebut que l'on rencontre avec des réactifs radioactifs. Les marqueurs préférés sont des colorants au difluorure de dipyrrométhènebore (4,4-difluoro-4-bora-3a,4a-diaza-s-indacène).

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