Methods for the detection, analysis and isolation of nascent...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/00 (2006.01) C07K 1/13 (2006.01) C12N 15/10 (2006.01) C40B 50/06 (2006.01) G01N 1/00 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2512552

This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell- free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4~difluoro-4-bora-3a,4a-diaza-s- indacene) dyes.

L'invention porte sur des marqueurs non radioactifs facilitant la détection et l'analyse de protéines naissantes qui ainsi marquées peuvent être rapidement et efficacement détectées, isolées et analysées, sans connaître les problèmes dus à la manipulation et à l'élimination des réactifs radioactifs. Les marqueurs préférés sont des colorants du type difluorure de dipyrrométhénébore (4,4-difluoro-4-bora-3a,4a-diaza-s-indacène).

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