Methods for the detection and removal of mutant sequences...

Details Methods for the detection and removal of mutant sequences... Methods for the detection and removal of mutant sequences...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2240346

Method for removing DNA molecules containing one or more polymerase-generated mutations in a population of enzymatically amplified DNA duplexes comprising the steps of denaturing and reannealing the population of DNA duplexes, contacting the reannealled DNA duplexes with a mismatch repair system such that each strand is cleaved in DNA duplexes containing a base pair mismatch, and separating the cleaved DNA duplexes from uncleaved DNA duplexes.

Méthode d'extraction de molécules d'ADN contenant une ou plusieurs mutations résultant d'une polymérase dans une population de duplexes d'ADN amplifiés de manière enzymatique, cette méthode prévoyant les étapes consistant à dénaturer puis reconstituer la population de duplexes d'ADN, soumettre les duplexes d'ADN reconstitués au contact d'un système de rétablissement des mésappariements de telle sorte que chaque souche soit clivée dans les duplexes d'ADN présentant un mésappariement de paire de base, puis à séparer les duplexes d'ADN clivés des duplexes d'ADN non clivés.

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