Methods for the diagnosis of diabetes and prediabetic...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/88 (2006.01) G01N 33/564 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2155677

A method for detecting a autoantibodies to glutamic acid decarboxylase (GAD) in the serum of a patient as diagnostic of a diabetic or prediabetic condition in the patient, comprises contacting a serum sample from the patient with a GAD antigen and detecting binding of autoantibodies to GAD in the sample by the GAD antigen, wherein the GAD antigen comprises a GAD preparation containing an enhanced amount of dimer(s) or oligomer(s) of the 65 kD and/or 67 kD isoforms of GAD. A diagnostic kit is also disclosed.

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