Methods for the elimination of dna sequencing artifacts

Details Methods for the elimination of dna sequencing artifacts Methods for the elimination of dna sequencing artifacts

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2181266

The present invention relates to improvements in methods of DNA sequencing. In particular, the invention relates to the elimination of stops or pauses in chain termination methods of DNA sequencing by the addition of nitrogen-containing organic compounds such as betaine, trimethylamine N-oxide and dimethylglycine. The invention also provides for DNA sequencing kits containing these compounds. The invention also provides for improvements in other laboratory procedures using DNA polymerases, such as polymerase chain reaction (PCR).

L'invention concerne des procédés améliorés de séquençage d'ADN. Elle concerne, en particulier, l'élimination d'arrêts ou de pauses dans des procédés de séquençage d'ADN par terminaison de la chaîne réalisés par addition de composés organiques contenant de l'azote, tels que la bétaïne, le N-oxyde de triméthylamine et la diméthylglycine. Elle concerne également des trousses de séquençage d'ADN contenant lesdits composés. Elle concerne enfin des améliorations apportées à d'autres procédés de laboratoire selon lesquels on utilise des ADN polymérases, tels que la PCR.

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